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mirna microarray data analysis genechip mirna arrays  (Thermo Fisher)


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    Structured Review

    Thermo Fisher mirna microarray data analysis genechip mirna arrays
    Mirna Microarray Data Analysis Genechip Mirna Arrays, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mirna+microarray+data+analysis/pm23723006-70-45-50
    Average 90 stars, based on 1 article reviews
    mirna microarray data analysis genechip mirna arrays - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Microarray:

    Article Title: MicroRNA-mediated dysregulation of neural developmental genes in HPRT deficiency: clues for Lesch-Nyhan disease?
    Article Snippet: .. MicroRNA arrays and data analysis NcodeTM human miRNA Microarray V3 (Invitrogen) was used for expression array analysis, using conditions recommended by the manufacturer (NCode human miRNA Microarray V3, http://invitrogen.com , catalog no. MIRH3-05). ..

    Article Title: The microRNA167 controls somatic embryogenesis in Arabidopsis through regulating its target genes ARF6 and ARF8
    Article Snippet: MicroRNAs (miRNAs) are single-stranded small, endogenous RNAs that can downregulate gene expression in plants and animals.. Plant miRNAs are already known to function in many biological processes during plant development by targeting mRNA for degradation or repression.. Somatic embryogenesis is used as a model to investigate miRNAs associated with embryogenesis and also as an important pathway for plant propagation.

    Article Title: Dorsal root ganglion-derived exosomes deteriorate neuropathic pain by activating microglia via the microRNA-16-5p/HECTD1/HSP90 axis
    Article Snippet: Briefly, total RNA from DRG-Exo- and PBS-treated microglia was extracted, labeled, and processed for hybridization with Agilent SurePrint mouse miRNA microarrays. .. Microarray data analysis was performed on the Affymetrix miRNA 4.0 platform (Santa Clara, CA, USA). ..

    Article Title: Relationship between downregulation of miRNAs and increase of oxidative stress in the development of diabetic cardiac dysfunction: junctin as a target protein of miR-1.
    Article Snippet: Oxidative stress is involved in the etiology of diabetes-induced cardiac dysfunction while microRNAs (miRNAs) are known as regulators for genes involved in cardiac remodeling.. However, a functional link between miRNAs and diabetes-induced cardiac dysfunction remains to be investigated.. Here, we aimed to identify whether the expression levels of miRNAs are associated with oxidative stress/diabetic heart and if proteins responsible from contractile activity during diabetes might be directly modulated by miRNAs.

    Article Title: MicroRNA-941 regulates the proliferation of breast cancer cells by altering histone H3 Ser 10 phosphorylation
    Article Snippet: .. Nagar, India , Tel.9779199966; Fax: +91-172-2214692 Email: tikoo.k@gmail.com Nano drop ND 1000 RIN VALUE≥6 Total RNA sample RNA integrity check on Agilent 2100 Bioanalyzer Labelling of quality analysed samples Hybridization on Affymetrix Gene Chip miRNA 4.0 array Scanning using Affymetrix microarray scanner 3000 7G Raw data generation Bioinformatic analysis Hybridization conditions: 48°C and 60 rpm for 16-18 h Supplementary File Supplementary Figure S1: Affymetrix Human miRNA profiling workflow Supplementary Figure S2: Bioinformatic Analysis Affymetrix Transcriptome Console tool DATA COLLECTION (. .. CEL FILES) Normalisation Data pre-processing, Quality control and Base line transformation DEG (Differentially expressed miRNA selection) Clustering of data and miRNA conditions Brief report Affymetrix Expression Console tool Fold change ≥ 2.0 Supplementary Figure S3: Scatter plot of differentially expressed miRNAs In the above figure, red colour shows up regulation of miRNAs & blue colour shows down regulation of miRNAs in TNBC cell line (MDA-MB 231) when compared to MCF10A cell line Supplementary Figure S4: Hierarchical clustering images of upregulated (S4Aup) and downregulated (S4B-down) miRNAs respectively Figure S4 shows heat maps of upregulated and downregulated miRNAs in MDA-MB-231 cell line when compared to MCF-10A cell line.

    Article Title: Deficiency of the purine metabolic gene HPRT dysregulates microRNA-17 family cluster and guanine-based cellular functions: a role for EPAC in Lesch-Nyhan syndrome.
    Article Snippet: .. MicroRNA arrays and data analysis NcodeTM human miRNA Microarray V3 (Life Technologies) was used for expression array analysis, under conditions recommended by the manufacturer (NCode human miRNA Microarray V3, http://invitrogen.com, Catalog #: MIRH3-05). ..

    Expressing:

    Article Title: MicroRNA-mediated dysregulation of neural developmental genes in HPRT deficiency: clues for Lesch-Nyhan disease?
    Article Snippet: .. MicroRNA arrays and data analysis NcodeTM human miRNA Microarray V3 (Invitrogen) was used for expression array analysis, using conditions recommended by the manufacturer (NCode human miRNA Microarray V3, http://invitrogen.com , catalog no. MIRH3-05). ..

    Article Title: Deficiency of the purine metabolic gene HPRT dysregulates microRNA-17 family cluster and guanine-based cellular functions: a role for EPAC in Lesch-Nyhan syndrome.
    Article Snippet: .. MicroRNA arrays and data analysis NcodeTM human miRNA Microarray V3 (Life Technologies) was used for expression array analysis, under conditions recommended by the manufacturer (NCode human miRNA Microarray V3, http://invitrogen.com, Catalog #: MIRH3-05). ..

    Extraction:

    Article Title: The microRNA167 controls somatic embryogenesis in Arabidopsis through regulating its target genes ARF6 and ARF8
    Article Snippet: MicroRNAs (miRNAs) are single-stranded small, endogenous RNAs that can downregulate gene expression in plants and animals.. Plant miRNAs are already known to function in many biological processes during plant development by targeting mRNA for degradation or repression.. Somatic embryogenesis is used as a model to investigate miRNAs associated with embryogenesis and also as an important pathway for plant propagation.

    Isolation:

    Article Title: The microRNA167 controls somatic embryogenesis in Arabidopsis through regulating its target genes ARF6 and ARF8
    Article Snippet: MicroRNAs (miRNAs) are single-stranded small, endogenous RNAs that can downregulate gene expression in plants and animals.. Plant miRNAs are already known to function in many biological processes during plant development by targeting mRNA for degradation or repression.. Somatic embryogenesis is used as a model to investigate miRNAs associated with embryogenesis and also as an important pathway for plant propagation.

    Agarose Gel Electrophoresis:

    Article Title: Relationship between downregulation of miRNAs and increase of oxidative stress in the development of diabetic cardiac dysfunction: junctin as a target protein of miR-1.
    Article Snippet: Oxidative stress is involved in the etiology of diabetes-induced cardiac dysfunction while microRNAs (miRNAs) are known as regulators for genes involved in cardiac remodeling.. However, a functional link between miRNAs and diabetes-induced cardiac dysfunction remains to be investigated.. Here, we aimed to identify whether the expression levels of miRNAs are associated with oxidative stress/diabetic heart and if proteins responsible from contractile activity during diabetes might be directly modulated by miRNAs.

    Hybridization:

    Article Title: MicroRNA-941 regulates the proliferation of breast cancer cells by altering histone H3 Ser 10 phosphorylation
    Article Snippet: .. Nagar, India , Tel.9779199966; Fax: +91-172-2214692 Email: tikoo.k@gmail.com Nano drop ND 1000 RIN VALUE≥6 Total RNA sample RNA integrity check on Agilent 2100 Bioanalyzer Labelling of quality analysed samples Hybridization on Affymetrix Gene Chip miRNA 4.0 array Scanning using Affymetrix microarray scanner 3000 7G Raw data generation Bioinformatic analysis Hybridization conditions: 48°C and 60 rpm for 16-18 h Supplementary File Supplementary Figure S1: Affymetrix Human miRNA profiling workflow Supplementary Figure S2: Bioinformatic Analysis Affymetrix Transcriptome Console tool DATA COLLECTION (. .. CEL FILES) Normalisation Data pre-processing, Quality control and Base line transformation DEG (Differentially expressed miRNA selection) Clustering of data and miRNA conditions Brief report Affymetrix Expression Console tool Fold change ≥ 2.0 Supplementary Figure S3: Scatter plot of differentially expressed miRNAs In the above figure, red colour shows up regulation of miRNAs & blue colour shows down regulation of miRNAs in TNBC cell line (MDA-MB 231) when compared to MCF10A cell line Supplementary Figure S4: Hierarchical clustering images of upregulated (S4Aup) and downregulated (S4B-down) miRNAs respectively Figure S4 shows heat maps of upregulated and downregulated miRNAs in MDA-MB-231 cell line when compared to MCF-10A cell line.



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    GraphPad Software Inc mirna microarray data analysis
    (A)Volcano plot of <t>miRNA</t> <t>expression</t> in RV-HF vs RV-Ctrl. Blue dots, miRNAs that were differentially expressed at P<0.10. Labeled dots, miRNAs that were differentially expressed at a minimum 2-fold change in either direction (n=3 per group). (B)Heat maps, Venn diagram, and summary bar graph of differentially expressed miRNAs. Orange font, differentially expressed in RV-HF vs RV-Ctrl and in LV-HF vs LV-Ctrl but not statistically significantly different in RV-HF vs LV-HF. Blue font, differentially expressed in RV-HF vs RV-Ctrl and in RV-HF vs LV-HF, but not statistically significantly different in LV-HF vs LV-Ctrl. Purple font, differentially expressed across all three comparisons: LV-HF vs LV-Ctrl, RV-HF vs RV-Ctrl, and RV-HF vs LV-HF. *P<0.05 vs respective LV-HF/LV-Ctrl. (C)Quantitative RT-PCR analysis of miR-21 and miR-221 in ventricular tissue, n= 6 per group. *P<0.01 vs respective Ctrl; #P<0.01 vs LV HF. (D)Cyclic overstretch and/or aldosterone induced a marked increase in miR-21 (*P<0.01 vs unstimulated) and (E)miR-221 (*P<0.05 vs unstimulated) only in RV fibroblasts. (F)Inhibition of miR-21/−221 attenuated proliferation in RV but not LV fibroblasts. n= 4 per experimental condition. *P<0.05 vs respective LV, #P<0.05 vs RV without antimir, analyzed by ANOVA on Ranks.
    Mirna Microarray Data Analysis, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mirna+microarray+data+analysis/mirna+microarray+data+analysis/pmc07006717-91-7-27
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    Thermo Fisher mirna microarray data analysis genechip mirna arrays
    (A)Volcano plot of <t>miRNA</t> <t>expression</t> in RV-HF vs RV-Ctrl. Blue dots, miRNAs that were differentially expressed at P<0.10. Labeled dots, miRNAs that were differentially expressed at a minimum 2-fold change in either direction (n=3 per group). (B)Heat maps, Venn diagram, and summary bar graph of differentially expressed miRNAs. Orange font, differentially expressed in RV-HF vs RV-Ctrl and in LV-HF vs LV-Ctrl but not statistically significantly different in RV-HF vs LV-HF. Blue font, differentially expressed in RV-HF vs RV-Ctrl and in RV-HF vs LV-HF, but not statistically significantly different in LV-HF vs LV-Ctrl. Purple font, differentially expressed across all three comparisons: LV-HF vs LV-Ctrl, RV-HF vs RV-Ctrl, and RV-HF vs LV-HF. *P<0.05 vs respective LV-HF/LV-Ctrl. (C)Quantitative RT-PCR analysis of miR-21 and miR-221 in ventricular tissue, n= 6 per group. *P<0.01 vs respective Ctrl; #P<0.01 vs LV HF. (D)Cyclic overstretch and/or aldosterone induced a marked increase in miR-21 (*P<0.01 vs unstimulated) and (E)miR-221 (*P<0.05 vs unstimulated) only in RV fibroblasts. (F)Inhibition of miR-21/−221 attenuated proliferation in RV but not LV fibroblasts. n= 4 per experimental condition. *P<0.05 vs respective LV, #P<0.05 vs RV without antimir, analyzed by ANOVA on Ranks.
    Mirna Microarray Data Analysis Genechip Mirna Arrays, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    LC Sciences mirna microarray data analysis
    (A)Volcano plot of <t>miRNA</t> <t>expression</t> in RV-HF vs RV-Ctrl. Blue dots, miRNAs that were differentially expressed at P<0.10. Labeled dots, miRNAs that were differentially expressed at a minimum 2-fold change in either direction (n=3 per group). (B)Heat maps, Venn diagram, and summary bar graph of differentially expressed miRNAs. Orange font, differentially expressed in RV-HF vs RV-Ctrl and in LV-HF vs LV-Ctrl but not statistically significantly different in RV-HF vs LV-HF. Blue font, differentially expressed in RV-HF vs RV-Ctrl and in RV-HF vs LV-HF, but not statistically significantly different in LV-HF vs LV-Ctrl. Purple font, differentially expressed across all three comparisons: LV-HF vs LV-Ctrl, RV-HF vs RV-Ctrl, and RV-HF vs LV-HF. *P<0.05 vs respective LV-HF/LV-Ctrl. (C)Quantitative RT-PCR analysis of miR-21 and miR-221 in ventricular tissue, n= 6 per group. *P<0.01 vs respective Ctrl; #P<0.01 vs LV HF. (D)Cyclic overstretch and/or aldosterone induced a marked increase in miR-21 (*P<0.01 vs unstimulated) and (E)miR-221 (*P<0.05 vs unstimulated) only in RV fibroblasts. (F)Inhibition of miR-21/−221 attenuated proliferation in RV but not LV fibroblasts. n= 4 per experimental condition. *P<0.05 vs respective LV, #P<0.05 vs RV without antimir, analyzed by ANOVA on Ranks.
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    Thermo Fisher data analysis ncode human mirna microarray v3
    (A)Volcano plot of <t>miRNA</t> <t>expression</t> in RV-HF vs RV-Ctrl. Blue dots, miRNAs that were differentially expressed at P<0.10. Labeled dots, miRNAs that were differentially expressed at a minimum 2-fold change in either direction (n=3 per group). (B)Heat maps, Venn diagram, and summary bar graph of differentially expressed miRNAs. Orange font, differentially expressed in RV-HF vs RV-Ctrl and in LV-HF vs LV-Ctrl but not statistically significantly different in RV-HF vs LV-HF. Blue font, differentially expressed in RV-HF vs RV-Ctrl and in RV-HF vs LV-HF, but not statistically significantly different in LV-HF vs LV-Ctrl. Purple font, differentially expressed across all three comparisons: LV-HF vs LV-Ctrl, RV-HF vs RV-Ctrl, and RV-HF vs LV-HF. *P<0.05 vs respective LV-HF/LV-Ctrl. (C)Quantitative RT-PCR analysis of miR-21 and miR-221 in ventricular tissue, n= 6 per group. *P<0.01 vs respective Ctrl; #P<0.01 vs LV HF. (D)Cyclic overstretch and/or aldosterone induced a marked increase in miR-21 (*P<0.01 vs unstimulated) and (E)miR-221 (*P<0.05 vs unstimulated) only in RV fibroblasts. (F)Inhibition of miR-21/−221 attenuated proliferation in RV but not LV fibroblasts. n= 4 per experimental condition. *P<0.05 vs respective LV, #P<0.05 vs RV without antimir, analyzed by ANOVA on Ranks.
    Data Analysis Ncode Human Mirna Microarray V3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (A)Volcano plot of miRNA expression in RV-HF vs RV-Ctrl. Blue dots, miRNAs that were differentially expressed at P<0.10. Labeled dots, miRNAs that were differentially expressed at a minimum 2-fold change in either direction (n=3 per group). (B)Heat maps, Venn diagram, and summary bar graph of differentially expressed miRNAs. Orange font, differentially expressed in RV-HF vs RV-Ctrl and in LV-HF vs LV-Ctrl but not statistically significantly different in RV-HF vs LV-HF. Blue font, differentially expressed in RV-HF vs RV-Ctrl and in RV-HF vs LV-HF, but not statistically significantly different in LV-HF vs LV-Ctrl. Purple font, differentially expressed across all three comparisons: LV-HF vs LV-Ctrl, RV-HF vs RV-Ctrl, and RV-HF vs LV-HF. *P<0.05 vs respective LV-HF/LV-Ctrl. (C)Quantitative RT-PCR analysis of miR-21 and miR-221 in ventricular tissue, n= 6 per group. *P<0.01 vs respective Ctrl; #P<0.01 vs LV HF. (D)Cyclic overstretch and/or aldosterone induced a marked increase in miR-21 (*P<0.01 vs unstimulated) and (E)miR-221 (*P<0.05 vs unstimulated) only in RV fibroblasts. (F)Inhibition of miR-21/−221 attenuated proliferation in RV but not LV fibroblasts. n= 4 per experimental condition. *P<0.05 vs respective LV, #P<0.05 vs RV without antimir, analyzed by ANOVA on Ranks.

    Journal: Circulation. Heart failure

    Article Title: Differential microRNA-21 and microRNA-221 upregulation in the biventricular failing heart reveals distinct stress responses of right versus left ventricular fibroblasts

    doi: 10.1161/CIRCHEARTFAILURE.119.006426

    Figure Lengend Snippet: (A)Volcano plot of miRNA expression in RV-HF vs RV-Ctrl. Blue dots, miRNAs that were differentially expressed at P<0.10. Labeled dots, miRNAs that were differentially expressed at a minimum 2-fold change in either direction (n=3 per group). (B)Heat maps, Venn diagram, and summary bar graph of differentially expressed miRNAs. Orange font, differentially expressed in RV-HF vs RV-Ctrl and in LV-HF vs LV-Ctrl but not statistically significantly different in RV-HF vs LV-HF. Blue font, differentially expressed in RV-HF vs RV-Ctrl and in RV-HF vs LV-HF, but not statistically significantly different in LV-HF vs LV-Ctrl. Purple font, differentially expressed across all three comparisons: LV-HF vs LV-Ctrl, RV-HF vs RV-Ctrl, and RV-HF vs LV-HF. *P<0.05 vs respective LV-HF/LV-Ctrl. (C)Quantitative RT-PCR analysis of miR-21 and miR-221 in ventricular tissue, n= 6 per group. *P<0.01 vs respective Ctrl; #P<0.01 vs LV HF. (D)Cyclic overstretch and/or aldosterone induced a marked increase in miR-21 (*P<0.01 vs unstimulated) and (E)miR-221 (*P<0.05 vs unstimulated) only in RV fibroblasts. (F)Inhibition of miR-21/−221 attenuated proliferation in RV but not LV fibroblasts. n= 4 per experimental condition. *P<0.05 vs respective LV, #P<0.05 vs RV without antimir, analyzed by ANOVA on Ranks.

    Article Snippet: miRNA microarray data was analyzed for differential miRNA expression between pre-specified groups (RV-HF vs. RV-Ctrl, LV-HF vs. LV-Ctrl, and RV-HF vs. LV-HF) by two-tailed Student’s t-test using GraphPad Software (Prism 7.0).

    Techniques: Expressing, Labeling, Quantitative RT-PCR, Inhibition